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| Souza,A.J.; Ferreira,A.T.S.; Perales,J.; Beghini,D.G.; Fernandes,K.V.S.; Xavier-Filho,J.; Venancio,T.M.; Oliveira,A.E.A.. |
| Seed coat is a specialized maternal tissue that interfaces the embryo and the external environment during embryogenesis, dormancy and germination. In addition, it is the first defensive barrier against penetration by pathogens and herbivores. Here we show that Albizia lebbeck seed coat dramatically compromises the oviposition, eclosion and development of the bruchid Callosobruchus maculatus. Dietary supplementation of bruchid larvae with A. lebbeck seed coat flour causes severe weight loss and reduces survival. By means of protein purification, mass spectrometry and bioinformatic analyses, we show that chitin-binding vicilins are the main source of A. lebbeck tegumental toxicity to C. maculatus. At concentrations as low as 0.1%, A. lebbeck vicilins reduce... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitin-binding proteins; Seed coat; Callosobruchus maculatus; Insect bruchid. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200005 |
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| Azevedo,C.R.; Maciel,F.M.; Silva,L.B.; Ferreira,A.T.S.; da Cunha,M.; Machado,O.L.T.; Fernandes,K.V.S.; Oliveira,A.E.A.; Xavier-Filho,J.. |
| Evidence based on immunological cross-reactivity and anti-diabetic properties has suggested the presence of insulin-like peptides in plants. The objective of the present study was to investigate the presence of insulin-like proteins in the leaves of Bauhinia variegata ("pata-de-vaca", "mororó"), a plant widely utilized in popular medicine as an anti-diabetic agent. We show that an insulin-like protein was present in the leaves of this plant. A chloroplast protein with a molecular mass similar to that of bovine insulin was extracted from 2-mm thick 15% SDS-PAGE gels and fractionated with a 2 x 24 cm Sephadex G-50 column. The activity of this insulin-like protein (0.48 mg/mL) on serum glucose levels of four-week-old Swiss albino (CF1) diabetic mice was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bauhinia variegata; Plant insulin; Chloroplast; Medicinal plant. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100007 |
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| Moraes,R.A.; Sales,M.P.; Pinto,M.S.P.; Silva,L.B.; Oliveira,A.E.A.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.. |
| The presence of phaseolin (a vicilin-like 7S storage globulin) peptides in the seed coat of the legume Phaseolus lunatus L. (lima bean) was demonstrated by N-terminal amino acid sequencing. Utilizing an artificial seed system assay we showed that phaseolin, isolated from both cotyledon and testa tissues of P. lunatus, is detrimental to the nonhost bruchid Callosobruchus maculatus (F) (cowpea weevil) with ED50 of 1.7 and 3.5%, respectively. The level of phaseolin in the seed coat (16.7%) was found to be sufficient to deter larval development of this bruchid. The expression of a C. maculatus-detrimental protein in the testa of nonhost seeds suggests that the protein may have played a significant role in the evolutionary adaptation of bruchids to legume seeds. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lima bean; Phaseolin (vicilin); 7S storage globulin; Seed coat; Cowpea weevil; Resistance to bruchids. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200005 |
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| Silva,L.B.; Santos,S.S.S.; Azevedo,C.R.; Cruz,M.A.L.; Venâncio,T.M.; Cavalcante,C.P.; Uchôa,A.F.; Astolfi Filho,S.; Oliveira,A.E.A.; Fernandes,K.V.S.; Xavier-Filho,J.. |
| We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insulin; Insulin-like; Antigens; Evolution; Plants; Cyanobacteria; Algae; Fungi. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300004 |
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